Loading...

Description

Caloric restriction (CR) stimulates development of functional beige fat and extends healthy lifespan. Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning. Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component. The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals. Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations. These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.

Summary

Project accession BioProject accession Keywords PMID #Samples
SRP157514 PRJNA480387 Feces Cecum C57BL/6 Charles River Laboratories Laboratory 30174308 53

Alpha diversity

Samples

Sample accession Project accession Sampling location Genotype Vendor Origin
SRS3665243 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665242 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665241 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665239 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665236 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665233 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665229 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665228 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665227 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665226 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665219 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3665216 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665215 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665208 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3665206 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3665202 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3665200 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3665201 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3665199 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3665198 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3665197 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667910 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667908 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667907 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667905 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667904 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667903 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667902 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667900 SRP157514 Cecum C57BL/6 Charles River Laboratories Laboratory
SRS3667894 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667893 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667890 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667885 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667884 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667883 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667882 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667880 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667875 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667874 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667871 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667870 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667869 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667868 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667867 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667866 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667865 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667864 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667862 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667863 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667852 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667853 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667851 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory
SRS3667850 SRP157514 Feces C57BL/6 Charles River Laboratories Laboratory