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Description

Diets rich in fruits and vegetables may reduce oxidative stress (OxS) and inflammation via several mechanisms. These beneficial effects may be due to their high polyphenol content. The aims of the present study are to evaluate the preventive and therapeutic aspects of polyphenols in dried apple peel powder (DAPP) on intestinal inflammation while elucidating the underlying mechanisms and clinical benefits. Induction of intestinal inflammation in mice was performed by oral administration of the inflammatory agent dextran sulfate sodium (DSS) at 2.5% for 10 days. Physiological and supraphysiological doses of DAPP (200 and 400 mg/kg/day respectively) were administered by gavage for 10 days pre- and post-DSS treatment. DSS-mediated inflammation caused weight loss, shortening of the colon, dystrophic detachment of the epithelium, and infiltration of mono- and poly-morphonuclear cells in the colon. DSS induced an increase in lipid peroxidation, a down-regulation of antioxidant enzymes, an augmented expression of myeloperoxidase (MPO) and cyclooxygenase-2 (COX-2), an elevated production of prostaglandin E2 (PGE2) and a shift in mucosa-associated microbial composition. However, DAPP normalized most of these abnormalities in preventive or therapeutic situations in addition to lowering inflammatory cytokines while stimulating antioxidant transcription factors and modulating other potential healing pathways. The supraphysiological dose of DAPP in therapeutic situations also improved mitochondrial dysfunction. Relative abundance of Peptostreptococcaceae and Enterobacteriaceae bacteria was slightly decreased in DAPP-treated mice. In conclusion, DAPP exhibits powerful antioxidant and anti-inflammatory action in the intestine and is associated with the regulation of cellular signalling pathways and changes in microbiota composition. Evaluation of preventive and therapeutic effects of DAPP may be clinically feasible in individuals with intestinal inflammatory bowel diseases.

Summary

Project accession BioProject accession Keywords PMID #Samples
SRP058488 PRJNA284368 Feces C57BL/6 Charles River Laboratories Laboratory 27630205 10

Alpha diversity

Samples

Sample accession Project accession Sampling location Genotype Vendor Origin
SRS940649 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS940678 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS940859 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS940871 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS941143 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS941212 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS941317 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS941318 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS941670 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory
SRS941681 SRP058488 Feces C57BL/6 Charles River Laboratories Laboratory