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SRP095440

A human stool-derived Bilophila wadsworthia strain caused systemic inflammation in specific-pathogen-free mice.

Description

BACKGROUND: Bilophila wadsworthia is a major member of sulfidogenic bacteria in human gut, it was originally recovered from different clinical specimens of intra-abdominal infections and recently was reported potentially linked to different chronic metabolic disorders. However, there is still insufficient understanding on its detailed function and mechanism to date. METHODS: A B. wadsworthia strain was isolated from fresh feces of a latent autoimmune diabetes in adults patient and we investigated its pathogenicity by oral administration to specific-pathogen-free mice. Tissue samples and serum were collected after sacrifice. Stool samples were collected at different time points to profile the gut microbiota. RESULTS: Bilophila wadsworthia infection resulted in the reduction of body weight and fat mass, apparent hepatosplenomegaly and elevated serum inflammatory factors, including serum amyloid A and interleukin-6, while without significant change of the overall gut microbiota structure. CONCLUSIONS: These results demonstrated that higher amount of B. wadsworthia caused systemic inflammatory response in SPF mice, which adds new evidence to the pathogenicity of this bacterium and implied its potential role to the chronic inflammation related metabolic diseases like diabetes.

Summary

Project accession BioProject accession Keywords PMID #Samples
SRP095440 PRJNA358144 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory 29090023 20

Alpha diversity

Samples

Sample accession Project accession Sampling location Genotype Vendor Origin
SRS1872570 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872565 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872566 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872568 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872567 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872569 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872571 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872572 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872573 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872576 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872579 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872580 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872586 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872584 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872587 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872588 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872589 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872596 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872597 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory
SRS1872600 SRP095440 Feces C57BL/6 Shanghai Laboratory Animal Center Laboratory

Reference

Feng, Zhou, et al. "A human stool-derived Bilophila wadsworthia strain caused systemic inflammation in specific-pathogen-free mice." Gut pathogens 9.1 (2017): 1-10.